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KMID : 1094719980030020115
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Biotechnology and Bioprocess Engineering
1998 Volume.3 No. 2 p.115 ~ p.118
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Ammonia production from yeast extract and its effect on growth of the hyperthermophilic archaeonSulfolobus solfataricus
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Park Chan-Beum
Lee Sun-Bok
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Abstract
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Utilization of yeast extract and formation of byproduct metabolite were investigated for hyperthermophilic archaeonSulfolobus solfataricus (DSM 1617). In both batch and fed-batch cultivations ofS. solfataricus, maximal cell density, NH4 + ion production and pH change were highly dependent on the ratio of yeast extract to glucose in the medium. Variation of NH4 + ion level was identified as a major cause of pH change during cultivation, and acidification of culture broth was attributed to consumption of NH4 + ions rather than formation of acid byproducts. It was also observed that increase of NH4 + ion concentrations in the medium resulted in greater degree of growth inhibition.
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KEYWORD
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hyperthermophile, archaea, Sulfolobus solfataricus, ammonia, yeast extract, fed-batch
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